Introduction: In the last 15 years, the most important revolution in dentistry has been the introduction and then the widespread adoption of the dental operating microscope. The aim of this study was to evaluate the usage of the dental operating microscope among young specialized and general practitioner dentists in Tîrgu Mureș. Material and Methods: A 9-point questionnaire about dental microscope usage was distributed among endodontists, practitioners of other specialties, and general practitioners with less than 10 years of experience. Results: According to our results, the dental operating microscope is used mainly during root canal therapy by endodontists. Practitioners of other specialties (prosthetists, alveolar dental surgeons, and orthodontists) use the microscope in a small percentage during coronal restoration, prosthetics, or periodontal surgery. Most general practitioners are not interested in using magnification in their practice. Conclusions: Endodontists use the dental operating microscope when they face complicated cases and have access to it. Working under magnification is awakening the interest of doctors of other specialties also. Private practices should invest in acquiring a microscope, and young general practitioners should be motivated in using it during dental treatments.